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土壤蔗糖酶(S-SC)檢測試劑盒

描述:測定意義:
S-SC能夠水解蔗糖變成相應(yīng)的單糖而被機(jī)體吸收,其酶促作用產(chǎn)物與土壤中有機(jī)質(zhì)、氮、磷含量,微生物數(shù)量及土壤呼吸強(qiáng)度密切關(guān),是評價(jià)土壤肥力的重要指標(biāo)。
測定原理:
S-SC催化蔗糖降解產(chǎn)生還原糖,進(jìn)一步與3,5-二硝基水楊酸反應(yīng),生成棕紅色氨基化合物,在510nm有特征光吸收,在一定范圍內(nèi)510nm光吸收增加速率與S-SC活性成正比。
土壤蔗糖酶(S-SC)檢測試劑盒

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  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2019-11-07
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產(chǎn)品詳情/ PRODUCT DETAIL

標(biāo)題:土壤蔗糖酶(S-SC)檢測試劑盒

產(chǎn)品概述

土壤蔗糖酶(S-SC)檢測試劑盒

自備用品:

可見分光光度計(jì)、臺(tái)式離心機(jī)、水浴鍋、可調(diào)式移液器、1mL玻璃比色皿、研缽、冰、甲苯(不允許快遞)和蒸餾水。

試劑組成和配制:                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

試劑一:甲苯5mL×1瓶,4保存;(自備)

試劑二:液體15mL×1瓶,4保存;

試劑三:粉劑×1瓶,4保存;臨用前每瓶加入40mL雙蒸水充分溶解備用;

試劑四:液體30mL×1瓶,4保存。

測定步驟和加樣表:

試劑名稱

測定管

對照管

風(fēng)干土樣(g

0.1

0.1

試劑一(μL

15

15

振蕩混勻,使土樣全部濕潤,37℃水浴15min

試劑二μL

250

250

試劑三μL

750

 

雙蒸水μL

 

750

充分混勻,放入37水浴培養(yǎng)24小時(shí),10000 g,4℃,離心5min,取上清液

上清液(μL    

200

200

試劑四(μL

500

500

充分混勻,放入沸水浴中煮沸5min(蓋緊,以防止水分散失),流水冷卻后充分混勻,蒸餾水稀釋10倍后(可以吸取100μL,加入900μL蒸餾水稀釋;如果吸光度大于4,可以適當(dāng)加大稀釋倍數(shù)),用對照管調(diào)零,510nm處記錄測定管吸光值A。

土壤蔗糖酶(S-SC)檢測試劑盒

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